Translational_Unit

Part:BBa_K1521000:Design

Designed by: Danilo Keiji Zampronio   Group: iGEM14_Brasil-SP   (2014-10-01)


Quorum threshold expression element (qteE) from Pseudomonas aeruginosa + SpoVg RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For appropriate use of this part as a threshold producer its important to test the molar ratio qteE:QSprotein in wich the inhibition caused by the QteE is on the verge of disappearing, so that you can set the threshold for your purpose correctly. If your putting qteE production under a inducible/repressible promoter it would be wise to do a concentration gradient for determination of the appropriate threshold.


Source

This sequence was amplified through PCR from the Pseudomonas Aeruginosa PA14 cDNA.

References